Journal: Nature nanotechnology

Article Title: Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma

doi: 10.1038/s41565-021-00939-8

Figure Lengend Snippet: a. CRISPR-FDS liposome assay signal detected with EVs isolated from 293F cells stably transfected with a SARS-CoV-2 N gene expression vector or the empty expression vector. Data represent the mean±SD of three replicates. b. CRISPR-FDS liposome assay kinetics upon analysis of EVs captured from 100 μL plasma aliquots of individuals diagnosed with and without COVID-19 (EV pos /EV neg samples) upon incubation with CRISPR-FDS reagent-loaded liposomes with or without polyethylene glycol (PEG), or with free CRISPR-FDS reagents. c. CRISPR-FDS liposome assay signal detected for plasma aliquots from 20 adults with COVID-19 and 10 adults without COVID-19, as diagnosed by nasal swab RT-qPCR results. d. CRISPR-FDS liposome assay signal detected for plasma samples from six patients diagnosed with COVID-19 based on (A40–A45) who were diagnosed COVID-19 negative by nasal swab sample and a healthy donor (HD) and positive control (PC, whose nasal swab samples are RT-qPCR positive). Data represent the mean±SD of three replicates. ****, p<0.0001.

Article Snippet: This material was then mixed with CRISPR-FDS reagents (10 μL RT enzyme, 300 μL 10× NEBuffer 2.1, 0.3 pmol N gene primer pairs, 10 tubes of TwistAmp ™ Basic powder, 300 μL rehydration buffer, 0.16 pmol Cas12a, 0.16 pmol N gene guide RNA, and 1 pmol FAM-labeled DNA probe).

Techniques: CRISPR, Isolation, Stable Transfection, Transfection, Expressing, Plasmid Preparation, Incubation, Quantitative RT-PCR, Positive Control

Journal: Intradermal Immunization

Article Title: Delivery Systems for Intradermal Vaccination

doi: 10.1007/82_2011_123

Figure Lengend Snippet: Liquid jet and solid projectile injectors. a Jet injector (Biojector 2000) with ID spacer (white portion at end of syringe), used for investigational use only (Courtesy of BioJect). b Jet injector applied to the skin for injection (Courtesy of PharmaJet). c Epidermal powder immunization device for ID projectile injection (Courtesy of PowderMed)

Article Snippet: Fig. 2 Liquid jet and solid projectile injectors. a Jet injector (Biojector 2000) with ID spacer (white portion at end of syringe), used for investigational use only (Courtesy of BioJect). b Jet injector applied to the skin for injection (Courtesy of PharmaJet). c Epidermal powder immunization device for ID projectile injection (Courtesy of PowderMed)

Techniques: Injection